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Microbiology Lab Manual

Microbiology Lab Manual

2nd Edition

Karen Messley, Stephen Norrell

May 2003, Paperback, 288 pages
ISBN13: 9780130100290
ISBN10: 0130100293
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For freshman/sophomore courses in General Introductory Microbiology, and Microbiology for Allied Health Students.

This laboratory manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment. Content covers not only basic lab instructions, but background material that explains the tests, the basis of results and their applications, providing a "procedural" aspect in addition to laboratory guidance. Questions are provided with each exercise to reinforce and challenge students' understanding of basic concepts and to require students to analyze or apply the material under discussion.


1. Introduction to Microscopy.
2. Preparation of Smears and Simple Stains.
3. Wet Mounts and Hanging Drops.
4. The Negative Stain.
5. Differential Staining Procedures: Gram Stain, Acid-Fast Stain.
6. Additional Differential Staining Procedures: Metachromatic-Granules, Capsule, Endospore and Flagella Stains.


7. Ubiquity of Microorganisms.
8. Survey of Microorganisms.


9. Isolation Techniques: Streak Plate.
10. Isolation Techniques: Pour Plates.
11. Oxygen Requirements of Microorganisms.
12. Physical Growth Requirements.
13. Selective and Differential Media.
14. Physiological Characteristics of Bacteria: Carbohydrate Metabolism.
15. Physiologic Characteristics of Bacteria: Reactions of Nitrogen Metabolism.
16. Physiologic Characteristics of Bacteria: Miscellaneous Reactions.
17. Multiple Test Media.


18. Development and Use of Diagnostic Keys.
19. Use of Diagnostic Keys: Bacterial Unknowns.
20. Rapid Diagnostic Tests: Profile Analysis.


21. Microbial Population Counts: Microscopic Methods.
22. Microbial Population Counts: Viable Cell Counts.


23. Physical Control Methods.
24. UV Light as an Antimicrobial Agent.
25. Assay of Antimicrobial Agents: Disc-Diffusion Methods.
26. Assay of Antimicrobial Agents: Use-Dilution Methods.
27. Antibiotic Sensitivity Testing: The Kirby-Bauer Procedure.
28. Detection of Mutant Strains of Bacteria.


29. Epidemiology.
30. Serological Reactions.
31. The Collection and Transport of Clinical Specimen.
32. Throat Cultures.
33. Urinary Tract Cultures.
34. Gastrointestinal Tract Cultures.
35. Lactobacillus Activity.


36. Viral Population Counts: Plaque Counting and Plaque Morphology.


37. Food Microbiology.
38. Water Analysis: Standard Methods.
39. Water Analysis: Membrane Filter Techniques.
Appendix A: Writing a Lab Report.
Appendix B: Cultures Used.
Appendix C: Media, Stains, Reagents, and Supplies.

  • NEW - Reorganized multi-part procedures—Complex exercises have been separated into individual activities.
    • Provides students with an easier organization and procedure to follow in the lab.

  • Self-contained exercises—Include explanations of the salient points being demonstrated or tested.
    • Provides students with exercises that can serve as stand-alone labs, and a manual that will supplement and compliment any standard text.

  • Consistent sequence to each exercise—i.e., Overview; Background Information; Lab Objectives; Materials Needed; Lab Procedures; and Lab Report Form.
    • Prepares students to complete the lab with minimal additional instruction, and makes it easy for them to refer back to procedural content when studying for lab or lecture exams.

  • Flexible application of exercises—Makes them adaptable for use over various laboratory time periods.
    • Allows instructors extensive flexibility in scheduling without having to modify instructions. Enables students to adjust to any prescribed modifications with minimal confusion.

  • Logical progression to complexity of exercises—While the instructions get less comprehensive.
    • Sets the expectation that students will become more competent with each exercise, and able to problem-solve some of the needed procedures for themselves.

  • Critical thinking exercises in laboratory reports—Includes questions based on the experimental procedures and principles demonstrated in each exercise.
    • Requires students to tabulate data acquired in the exercises and critically apply information from the procedures and results to other situations.